Coding

Part:BBa_K1129019:Design

Designed by: UBC iGEM 2013   Group: iGEM13_British_Columbia   (2013-08-30)


Complete caffeine synthesis pathway under pTET constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1373
    Illegal NheI site found at 1396
    Illegal NheI site found at 2757
    Illegal NheI site found at 2780
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2405
    Illegal BglII site found at 3807
    Illegal BglII site found at 3903
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have yet to test for expression, and we may find that codon optimization is needed.


Source

The constitutive promoter we used was from the 2012 Distribution Kit, part BBa_J23118. The caffeine biosynthesis genes we assembled were from the 2013 Distribution Kit and were submitted by the 2012 TU Munich team: parts BBa_K801070, BBa_K801071, BBa_K801072.

References